Words similar to cas
Example sentences for: cas
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The adaptor protein Cas has several domains that are important for its function, an SH3 domain, a central substrate domain with up to 15 tyrosines that can serve as tyrosine kinase substrates for upstream kinases such as Src and Abl [ 19 42 43 ] , and a C-terminal Src binding domain with PXXP motifs for binding to the SH3 domain of Src, and two tyrosines providing docking sites for the Src SH2 domain upon phosphorylation [ 6 ] . The substrate domain of Cas has two major types of tyrosine motifs that can be phosphorylated and bind downstream effectors, YQXP (4 copies) and YDXP motifs (9 copies), suggesting that at least two classes of downstream effectors, one capable of binding to YDXP motifs and the other capable of binding to YQXP motifs, are assembled by this adaptor protein.
Tyrosine phosphorylation of Cas is regulated by adhesion, such that adherent and migrating cells have high levels of tyrosine phosphorylated Cas, whereas cells in suspension have reduced levels of Cas phosphorylation [ 10 33 ] . In addition, as mentioned above, transformed cells that are able to grow in an anchorage independent manner show increased tyrosine phosphorylation of Cas [ 10 33 ] . These data suggest that tyrosine phosphorylation of Cas in part regulates a switch from adhesion dependent growth to adhesion independent growth.
These data suggest that the kinase inactive fusion with the Cas SD is either transiently tyrosine phosphorylated in cells and v-crk protects them from being dephosphorylated by interacting with them via its SH2 domain, or alternatively, that v-crk induces their tyrosine phosphorylation.
The v-crk SH2 domain interacts with YXXP motifs upon phosphorylation, suggesting that both of these regions may be able to interact with v-crk [ 37 ] . Fusions of the kinase inactive Src kinase domain and either the full Cas SD, the 5' region of the Cas SD (Cas(5'SD), containing 4 YQXP motifs) or the 3' of the Cas SD (Cas(3'SD), containing 9 YDXP motifs), are not stably tyrosine phosphorylated when expressed alone in cells (data not shown and see Fig.
Initially, we expressed the Src KM/Cas(SD) (entire Cas SD) in Cos-7 cells alone, or along with wild type (WT) v-crk, or v-crk SH2 or SH3 mutants and assayed for tyrosine phosphorylation and association by co-immunoprecipitation (Fig.
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