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Example sentences for: carpinteria
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Monoclonal antibodies were purchased from commercial vendors unless otherwise stated: CD3, IgG1; CD68, IgG1; CD34, IgG1; CD45, IgG1, D105, IgG1; and IgG2a controls (Dako Corporation, Carpinteria, CA, USA); anti-HLA-DR, IgG2a; CD14, IgG2b; CD34, IgG1 (Becton Dickinson, San Jose, CA, USA); anti-vimentin, IgG1; IgG2b control (Serotec, Kidlington, Oxfordshire, UK); anti-VCAM-1, IgG1; anti-αvβ3 (vitronectin receptor), IgG1 (Pharmingen, San Diego, CA, USA); anti-collagen-type-1, IgG1 (Sigma Diagnostics); anti-osteocalcin, IgG1 (ABOC-5021, Haematologic Technologies, Essex Junction, VT, USA) anti-IgG1.
The TYRP-1 and TSP-1 antibody reactions were optimized using the DAKO LSAB plus kit (DAKO, Carpinteria, CA, USA) for both frozen and FFPE sections.
Another set of sections was similarly incubated with anti-human ERα monoclonal antibody (ready to use without additional dilution), clone ID5 (DAKO Corporation, Carpinteria, CA).
The sections were pretreated for antigen retrieval using 'Dako pH 6.0 target retrieval solution' ®(Dako Corporation, Carpinteria, CA) at 95-100°C for 35 minutes and cooled at room temperature for 20 minutes.
Sections were processed for antibody staining using the following antibodies: rabbit [ 86 ] and sheep (BioDesign) anti-SOD1 (both recognize mouse and human SOD1), mouse anti-cytochrome c oxidase subunit CO1 and CO4 (Molecular Probes, OR), rabbit anti-TOM20 and TOM40 (Santa Cruz Biotechnology, CA), mouse anti-cytochrome c (BD PharMingen, San Diego, CA), sheep anti-catalase (The Binding Site, Birmingham, England), rabbit anti-catalase (Calbiochem, SanDiego, CA), rabbit anti-PMP70 (ABR, Golden, CO or Axxora, SanDiego, CA), and rabbit anti-cathepsin D (DAKO, Carpinteria, CA) according to the protocol described previously [ 26 28 ] . The stained sections were examined and digitized using a confocal microscope (Leica TCS-SP).
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