Words similar to carpinteria
Example sentences for: carpinteria
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The TYRP-1 and TSP-1 antibody reactions were optimized using the DAKO LSAB plus kit (DAKO, Carpinteria, CA, USA) for both frozen and FFPE sections.
Monoclonal antibodies were purchased from commercial vendors unless otherwise stated: CD3, IgG1; CD68, IgG1; CD34, IgG1; CD45, IgG1, D105, IgG1; and IgG2a controls (Dako Corporation, Carpinteria, CA, USA); anti-HLA-DR, IgG2a; CD14, IgG2b; CD34, IgG1 (Becton Dickinson, San Jose, CA, USA); anti-vimentin, IgG1; IgG2b control (Serotec, Kidlington, Oxfordshire, UK); anti-VCAM-1, IgG1; anti-αvβ3 (vitronectin receptor), IgG1 (Pharmingen, San Diego, CA, USA); anti-collagen-type-1, IgG1 (Sigma Diagnostics); anti-osteocalcin, IgG1 (ABOC-5021, Haematologic Technologies, Essex Junction, VT, USA) anti-IgG1.
Universal DAKO LSAB ®2 Peroxidase Kit (DAKO Corporation, Carpinteria, CA) was used according to the instructions provided by vendor with some modifications.
Secondary antibodies used: anti-mouse and anti-rabbit horseradish peroxidase, anti-mouse Texas Red (Amersham: Piscataway, NJ) and anti-mouse FITC (DAKO: Carpinteria, CA).
Parallel sections were stained with the MIB-1 mouse monoclonal antibody against Ki-67 (DAKO, Carpinteria, California).