Words similar to carlsbad

Example sentences for: carlsbad

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Samples of total RNA (2 μg) from rat livers were reverse transcribed according to the SuperScript' First-Strand Synthesis System (Invitrogen, Carlsbad, CA) using random hexamers with a final volume of 20 μl.
1-TOPO (Invitrogen, Carlsbad CA).
1 (Invitrogen, Carlsbad, CA).
Following extraction, we first amplified the picornavirus RNA using primers OL26 (5'-GCA CTT CTG TTT CCC C-3') [ 33 ] and OL27 (5'-CGG ACA CCC AAA GTA G-3') [ 33 ] using the Invitrogen SuperScript One-Step RT-PCR with PLATINUM Taq Kit (Invitrogen Corp., Carlsbad, California).
RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).