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Example sentences for: carlsbad

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Genomic and cDNA sources used as templates for amplification were obtained from commercial sources: RP-11 BACs from BACPAC Resources (Oakland, CA), YACs from the Whitehead Institute (Wl) (Cambridge, MA), and Marathon cDNA from Invitrogen Life Technologies (Carlsbad, CA).
This facility houses a custom made spotter built by Robotic Labware Designs (Carlsbad, CA).
Filarial DNA was extracted using a DNAzol (Invitrogen, Carlsbad, CA, USA) extraction method as described by the manufacturer.
Amplification products generated with Pwo polymerase were cloned using the Zeroblunt system, while products generated with Taq were cloned into pCRII (Invitrogen, Carlsbad, CA).
5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.