Example sentences for: carlsbad

How can you use “carlsbad” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.

  • cDNA was synthesized with oligo (dT)12-18 using Superscript First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA).

  • This PCR product was subsequently used as template DNA in a labeling reaction using the Radprime DNA labeling system (Invitrogen, Carlsbad, CA).

  • The amplified DNA was cloned into the TA cloning vector pCRII (Invitrogen, Carlsbad, CA) and then sequenced by the Sanger method using the Sequenase (Amersham Life Science, Cleveland, OH) protocol as described previously [ 37].

  • This facility houses a custom made spotter built by Robotic Labware Designs (Carlsbad, CA).


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