Words similar to carlsbad

Example sentences for: carlsbad

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The lysates were rocked in the cold for 1 h and centrifuged for 10 min at 10,000 × g . In some cases, a highly enriched mitochondrial fraction was obtained using a commercially available kit (Mitchondrial Fractionation Kit, Active Motif, Carlsbad, CA) according to the manufacturer's instructions.
Briefly, tissues were homogenized in CHAPS buffer 10 mM (3-[3-Cholamidropropyl) dimehthylammonio]-1-propane-sulfonate, 2 mM EDTA, 4 mM iodoacetate in phosphate buffered saline (pH 7.2) with protease inhibitor cocktail (Invitrogen; Carlsbad, CA) and cleared tissue lysate prepared by centrifugation at 10,000 g for 15 minutes.
Radiation hybrid mapping was used to localize the MADS on human chromosomes using high-resolution Gene-Bridge 4 radiation hybrid panels (Research Genetics, Inc, Carlsbad, CA, USA).
The amplified DNA was cloned into the TA cloning vector pCRII (Invitrogen, Carlsbad, CA) and then sequenced by the Sanger method using the Sequenase (Amersham Life Science, Cleveland, OH) protocol as described previously [ 37].
1 (Invitrogen, Carlsbad, CA).