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Example sentences for: carlsbad

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The amplified DNA was cloned into the TA cloning vector pCRII (Invitrogen, Carlsbad, CA) and then sequenced by the Sanger method using the Sequenase (Amersham Life Science, Cleveland, OH) protocol as described previously [ 37].
Vitravene, from Isis Pharmaceuticals in Carlsbad, California, is used to treat cytomegalovirus infections in the eye for patients with HIV.
The lysates were rocked in the cold for 1 h and centrifuged for 10 min at 10,000 × g . In some cases, a highly enriched mitochondrial fraction was obtained using a commercially available kit (Mitchondrial Fractionation Kit, Active Motif, Carlsbad, CA) according to the manufacturer's instructions.
At the beginning of each experiment, chondrocytes or OA synovial fibroblasts were washed three times with Hanks balanced salt solution (Cellgro) to remove traces of serum, and placed in DMEM containing 0.2% lactalbumin hydrolysate (Invitrogen, Carlsbad, CA, USA).
Phosphorylated eIF2α was detected with antiserum specific for the phosphorylated protein (Research Genetics, Carlsbad CA).