Example sentences for: carlsbad

How can you use “carlsbad” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All experiments were performed after overnight equilibration with macrophage serum-free medium (GIBCO, San Diego, California, United States; Invitrogen, Carlsbad, California, United States) supplemented with 5 ng/ml GM-CSF.

  • 1 construct containing HA-tagged Smo or v5-epitope tagged β2AR (Invitrogen; Carlsbad, USA).

  • Briefly, the protocol was as follows: double-stranded cDNA was synthesized from total RNA, with Invitrogen Life Technologies SuperScript Choice system reagents (Invitrogen, Carlsbad, CA, USA).

  • Oligonucleotides were synthesized at a 50 nmol scale by Invitrogen/Life Technologies (Carlsbad, CA) or Operon Technologies (Alameda, CA), and resuspended in sterile milliQ water to a final concentration of 100 μM.

  • Total RNA was treated with DNase I, Amplification Grade (Invitrogen, Carlsbad, CA) to remove DNA contamination before cDNA synthesis.


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