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Example sentences for: carlsbad

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Genomic and cDNA sources used as templates for amplification were obtained from commercial sources: RP-11 BACs from BACPAC Resources (Oakland, CA), YACs from the Whitehead Institute (Wl) (Cambridge, MA), and Marathon cDNA from Invitrogen Life Technologies (Carlsbad, CA).
The gel-purified PCR products were cloned into either a pSTBlue-1 vector (Novagen, Madison, WI) or pCR 4-TOPO TA vector (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.
At the beginning of each experiment, chondrocytes or OA synovial fibroblasts were washed three times with Hanks balanced salt solution (Cellgro) to remove traces of serum, and placed in DMEM containing 0.2% lactalbumin hydrolysate (Invitrogen, Carlsbad, CA, USA).
Human TTP cDNA was generated by RT-PCR from THP-1 RNA and cloned into the pcDNA 3.1His-C vector (Invitrogen, Carlsbad, CA, USA), which encodes a histidine and epitope (EXPRESS-TAG) at the N-terminus of the protein.
5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.