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Example sentences for: carinii
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The remaining two flasks, designated infected (I), were each inoculated with an aliquot of P. carinii -infected lung supernatant containing 7 × 10 5 P. carinii organisms per ml in 30 ml of media.
Several PCR product bands were seen in the P. carinii -infected but not in the mock-infected lanes of the mRNA differential display gel.
After development of P. carinii infection (approximately six weeks after inoculation), rats were sacrificed, and lungs were removed and homogenized in minimal essential medium (MEM) (Life Technologies, Inc., Grand Island, New York) containing 10% fetal calf serum and 1% non-essential amino acids.
These two regions could not be well aligned and we could not predict a common structure with comparative analysis with ten Eucaryotic sequences in 1988 (see Figures 35-43 in [ 48 ] ). However, once a larger number of related Eucaryotic 23S-like rRNA sequences was determined, we reanalyzed these two regions and were able to align those regions to other related organisms ( e.g., S. cerevisiae with Schizosaccharomyces pombe, Cryptococcus neoformans, Pneumocystis carinii, Candida albicans, and Mucor racemosus ) and predict a secondary structure that is common for all of these rRNAs (see Figures 3and 6 in [ 52 ] ). While the secondary structures for the fungal 23S-like rRNAs are determined in these regions, the animal rRNAs were only partially solved.
This hypothesis implies that the expression of all or most of the genes encoding various components of the ATP synthase complex would be up-regulated in response to P. carinii infection.
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