Words similar to carbonate
Example sentences for: carbonate
How can you use “carbonate” in a sentence? Here are some example sentences to help you improve your vocabulary:
cDNA was lyophilized to dryness and resuspended in 4.5 μl of 0.1 M sodium carbonate pH 9.0 buffer.
Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.
Isopycnic fractionation in sucrose gradients was performed essentially as described [ 43 ] . Briefly, cells (one confluent T162 flask of HT1080 or NIH 3T3 cells expressing different CD98 constructs) were lysed in 2 ml 500 mM sodium carbonate buffer, pH 11, in the presence of protease inhibitors and passed through 25 gauge needle 5 times followed by 6 passages through 27 gauge needle.
The association of lime and bones is not accidental: Limestone (calcium carbonate, CaCO3) is nothing but the fossilized skeletons of tiny sea animals, and calcium is the principal ingredient of bone; when Shakespeare wrote, in The Tempest , that Of his bones are coral made, he stated a truer fact of nature than he may have known.
Examples of donor-acceptor combinations might include: simple sugars and/or amino acids or other organic molecules in the presence of oxygen or nitrate (organisms capable of heterotrophic respiration), hydrogen and ferric ions or oxidized iron minerals (chemoautotrophic hydrogen oxidizers), organic acids and ferric ions or oxidized iron minerals (heterotrophic iron reducers), bicarbonate/carbonate and trace minerals in the presence and absence of light (photosynthetic organisms), carbon dioxide and a reduced metal such as ferrous ions (chemoautotrophs), ammonium ion and bicarbonate/carbonate (ammonia oxidizers), etc.
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