Example sentences for: carbonate

How can you use “carbonate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was extracted from 100 mg of frozen lung using Tri-reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and the final product was resuspended in 20 μl diethylpyro carbonate depC H 2 O. RNA was quantified and samples with A 260 /A 280 >1.

  • Adjoining negative control wells were coated with 0.1 M carbonate buffer (pH 9.6) alone.

  • cDNA was lyophilized to dryness and resuspended in 4.5 μl of 0.1 M sodium carbonate pH 9.0 buffer.

  • Protein solutions and NHS-ester activated Cy3 and Cy5 solutions (Amersham PA23001 and PA25001) were prepared in a 0.1 M pH 8.0 sodium carbonate buffer.

  • A 5-35% discontinuous sucrose gradient was formed above (1 ml of 5% sucrose: 2 ml of 35% sucrose, both in MBS containing 250 mM sodium carbonate) and centrifuged at 45 000 rpm for 16-18 hrs in SW 55 Ti rotor (Beckman Instruments, Palo Alto, CA).


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