Words similar to carbonate

Example sentences for: carbonate

How can you use “carbonate” in a sentence? Here are some example sentences to help you improve your vocabulary:

Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.
Isopycnic fractionation in sucrose gradients was performed essentially as described [ 43 ] . Briefly, cells (one confluent T162 flask of HT1080 or NIH 3T3 cells expressing different CD98 constructs) were lysed in 2 ml 500 mM sodium carbonate buffer, pH 11, in the presence of protease inhibitors and passed through 25 gauge needle 5 times followed by 6 passages through 27 gauge needle.
Adjoining negative control wells were coated with 0.1 M carbonate buffer (pH 9.6) alone.
Pastes use calcium carbonate to the same end.
Total RNA was extracted from 100 mg of frozen lung using Tri-reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and the final product was resuspended in 20 μl diethylpyro carbonate depC H 2 O. RNA was quantified and samples with A 260 /A 280 >1.