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References Burciaga 1993; Castillo and Camarillo 1973; Greenwood 1966; Jackson 1939; MacLean 1977; Siegal 1994
Primary antibodies specific for the following proteins were used: monoclonal mouse anti-CD36 antibody, clone FA 6–152 (IgG) (Immunotech, Fullerton, California, United States), clone SMO (IgM) (Santa Cruz Biotechnology, Santa Cruz, California, United States), rabbit polyclonal anti-CD36 (Santa Cruz Biotechnology), rabbit polyclonal anti-aquaporin1, anti-aquaporin2, anti-Na/K/2Cl (Chemicon, Temecula, California, United States), rabbit polyclonal phospho38/MAPK and mouse monoclonal p38 (Cell Signaling Technology, Beverly, Massachusetts, United States), rabbit polyclonal p-src (Y418) (Biosource, Camarillo, California, United States), and mouse monoclonal anti-tubulin (Sigma, St. Louis, Missouri, United States).
Immunoreactive TNF-α in cell-free media supernates was determined using an ultrasensitive rat-specific ELISA for TNF-α purchased from Biosource International (Camarillo, CA) with a detection limit of 0.7 pg/ml.
Serum levels of hTNF-α were determined by ELISA at a 1:2 dilution according to the manufacturer's instructions (Biosource International, Camarillo, CA, USA).
T-cell proliferation was assessed on day 5 by [ 3H]thymidine incorporation [ 14 22 ] . Antigen (proteoglycan)-specific interferon-γ, IL-10, and IL-4 production by T cells was determined in media collected on day 4 using capture enzyme-linked immunosorbent assays (ELISAs) from BioSource International, Inc. (Camarillo, CA, USA).
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