Words similar to calyculin

Example sentences for: calyculin

How can you use “calyculin” in a sentence? Here are some example sentences to help you improve your vocabulary:

The cells were extracted for 5 min on ice with 200 μl of 2× detergent extraction buffer (2% of Triton X-100 or DOTMAC, 50 mM Tris-HCl, 10 mM EGTA, 200 mM NaCl, 2 mM MgCl 2 , protease inhibitor cocktail [20 μg/ml aprotinin, 20 μM E-64, 200 μM leupeptin, 200 μM p-amidinophenylmethanesulfonyl fluoride], 2 μM calyculin A, 10 mM sodium pyrophosphate, 2 μM staurosporine, 20 μM phalloidin, and 20 μg/ml taxol).
As mentioned earlier, inhibition studies using OA and calyculin A suggested the existence of a PP1-like activity in P. falciparum extracts [ 15 ] . However, our attempts to purify the native PfPP1 enzyme by chromatographic procedures resulted in only small amounts of activity, probably due to rapid inactivation during fractionation.
NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.
The suspensions were divided and incubated with or without 1 μM calyculin A, 1 μM staurosporine, or 100 μM pervanadate in 200 μl of PBS(+) for 10 min at 37°C.
NIH 3T3 cells were detached from the dishes in the presence of 1 μM calyculin A, but not staurosporine and pervanadate.