Words similar to cacodylate
Example sentences for: cacodylate
How can you use “cacodylate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Subsequently, they were washed 4 x 4 min with working buffer to remove unbound secondary antibody and rinsed 1 x in cacodylate buffer (0.
Steady state kinetic studies were performed on multiple preparations of wild type L1, and the resulting kinetic data are shown in Tables 3, 4, 5. When using nitrocefin as substrate and 50 mM cacodylate, pH 7.0, as buffer, wild-type L1 exhibited a k cat value of 38 ± 1 s -1and a K m value of 12 ± 1 μM.
A typical experiment consisted of 25 μM enzyme and 5 μM nitrocefin in 50 mM cacodylate buffer, pH 7.0 containing 100 μM ZnCl 2 , the reaction temperature was thermostated at 25°C, and the spectra were collected between 300 and 725 nm.
Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.
The cell pellet was fixed by adding 2.5% glutraldehyde for 4 h and the pellet was washed in sodium cacodylate buffer.