Example sentences for: cacodylate

How can you use “cacodylate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The L4 and L5 spinal nerve roots and lumbar spinal cords (a 2 mm segment centered at the L5 root entry level) were dissected out and post-fixed with 2% osmium tetroxide in 100 mM cacodylate buffer, pH 7.6.

  • For polyadenylate tailing at the 3' end of the cDNA, 5 μl tailing buffer (200 mmol/l potassium cacodylate [pH 7.2], 4 mmol/l CoCl 2 , 0.4 mmol/l DTT), 2 mmol/l dATP (Roche, Indianapolis, IN, USA), and 10 U terminal transferase (Roche) were added, and incubated at 37°C for 20 min, followed by heat inactivation at 65°C for 10 min.

  • Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.

  • To the pellet 1 ml of 2.5% glutraldehyde (made in 0.1 M sodium cacodylate buffer) was added and centrifuged at 1150 g for 10 min, supernatant was decanted.

  • The mitochondrial pellets were incubated with 1% osmium tetroxide (Electron Microscopy Sciences) in cacodylate buffer for one hour on ice, followed by rinsing 3 x 4 min in ice-cold double-distilled water.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast