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Example sentences for: burlingame

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Formalin fixed, paraffin embedded mouse brain sections were deparaffinized and antigen retrieval was performed for 1 minute in a pressure cooker with antigen unmasking solution (Vector Laboratories, Burlingame, CA).
The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).
After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.
Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Following three PB washes, tissue was then incubated in biotinylated goat-anti-rabbit-IgG (1:200, Vector Labs, Burlingame, CA) in 0.1% Tx100 in PB overnight at 4°C.