Words similar to burlingame

Example sentences for: burlingame

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Finally, chromosome spreads were counterstained with 4,6-diamino-2-phenylindole (DAPI, 0.5 μg/ml) in an antifade solution (Vectashield ®, Vector Laboratories, Inc., Burlingame, CA).
The ECL reagents were purchased from Amersham; Vectashield mounting medium from Vector Laboratories (Burlingame, CA); DC protein assay kit from Bio-Rad (Hercules, CA); Biocoat and Transwells from Fisher and Biocclusive bandage from Johnson&Johnson Medical (Arlington, TX).
In brief, after dewaxing, inactivating endogenous peroxidase activity and blocking cross-reactivity with normal serum (Vectastain Elite Kit; Vector, Burlingame, CA), the sections were incubated overnight at 4□ with a diluted solution of the primary antibodies (1:1000 for p53, 1:200 for iNOS and 1:100 for VEGF).
The samples were washed extensively in PBS and then incubated with biotinylated secondary antibodies (Vector Laboratories, Burlingame, CA).
Microarrays were washed and sequentially incubated with streptavidin phycoerythrin (Molecular Probes, Eugene, OR), biotinylated anti-streptavidin antibody (Vector Laboratories, Inc., Burlingame, CA), and streptavidin phycoerythrin on the Fluidic Station (Affymetrix, Santa Clara, CA).