Words similar to budding
Example sentences for: budding
How can you use “budding” in a sentence? Here are some example sentences to help you improve your vocabulary:
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
Rad26/Rad3-like complexes also exist in budding yeast and human.
Budding yeast lacking PPT1 do not exhibit obvious growth defects or detectable phenotypes [ 2 ] . Disruption of PPT1 together with one of the other known phosphatases in budding yeast also reveals no obvious phenotype [ 23 ] . Thus, if Ppt1p has an essential function in S. cerevisiae , more than one other phosphatase can also carry out this role.
In those days, we budding artistes found this doctrine a congenial one, since you could carry it out by getting drunk before lectures or going to breakfast stoned.
We and others have focused on genetic analysis in the budding yeast S. cerevisiae to understand the mechanism of NPC biogenesis.
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