Words similar to buckinghamshire

Example sentences for: buckinghamshire

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The reaction was terminated by aspiration of the medium and addition of 200 μl lysis buffer from the Biotrak™ cAMP Enzyme Immunoassay kit from Amersham Pharmacia Biotech (Buckinghamshire, England).
Membranes were washed with TBST and then incubated for 1 hour at room temperature with a secondary antibody followed by washing three times with TBST, immersed in ECL Plus western blotting detection system (Amersham Pharmacia Biotech, Buckinghamshire, England) for 1 min and then exposed to Kodak X-ray film (Rochester, NY).
The reaction was terminated by aspiration of the medium and addition of lysis buffer from the Biotrak™ cAMP Enzyme Immunoassay kit from Amersham Pharmacia Biotech (Buckinghamshire, England).
1% Tween and developed using enhanced chemiluminescence (Amersham Corp, Buckinghamshire, UK) for 5 min.
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).