Words similar to bss

Example sentences for: bss

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In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.
BSS1: 5'-GACGGTATCGATAAGCTT-3'; BBS2: 5'-CCGGGCTGCAGGAATTC-3'; and BSS4: 5'-GACGGTATCGATAAGCTT(N) 25 GAATTCCTGCAGCCCGG-3' where N is A, T, C, or G.
The oligonucleotides in the degenerate pool were composed of twenty-five random nucleotides (25-mer) in the middle flanked by a specific sequence BSS1 at one end and the complementary sequence of BSS2 at the other end.
Subsequently, the primer set BSS1 and BSS2 was used to amplify the recovered oligonucleotides by PCR.
The 25-mer sequences of individual site selected sequences from each fusion protein are shown in the BSS1-N 25 -BSS2 orientation (Figure 2).