Words similar to bss

Example sentences for: bss

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The oligonucleotides in the degenerate pool were composed of twenty-five random nucleotides (25-mer) in the middle flanked by a specific sequence BSS1 at one end and the complementary sequence of BSS2 at the other end.
The 25-mer sequences of individual site selected sequences from each fusion protein are shown in the BSS1-N 25 -BSS2 orientation (Figure 2).
BSS1: 5'-GACGGTATCGATAAGCTT-3'; BBS2: 5'-CCGGGCTGCAGGAATTC-3'; and BSS4: 5'-GACGGTATCGATAAGCTT(N) 25 GAATTCCTGCAGCCCGG-3' where N is A, T, C, or G.
In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.
Subsequently, the primer set BSS1 and BSS2 was used to amplify the recovered oligonucleotides by PCR.