Example sentences for: bss
How can you use “bss” in a sentence? Here are some example sentences to help you improve your vocabulary:
BSS1: 5'-GACGGTATCGATAAGCTT-3'; BBS2: 5'-CCGGGCTGCAGGAATTC-3'; and BSS4: 5'-GACGGTATCGATAAGCTT(N) 25 GAATTCCTGCAGCCCGG-3' where N is A, T, C, or G.
In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.
The 25-mer sequences of individual site selected sequences from each fusion protein are shown in the BSS1-N 25 -BSS2 orientation (Figure 2).
Subsequently, the primer set BSS1 and BSS2 was used to amplify the recovered oligonucleotides by PCR.
DNAs were purified by phenol/chloroform extraction, followed by alcohol precipitation, then were amplified by PCR using the BSS1 and BSS2 primer set.