Example sentences for: bsa

How can you use “bsa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After another wash with 1 ml 0.5% Tween-20/1% BSA/PBS, nuclei were resuspended in 0.25 ml PBS containing 5 μg/ml Propidium Iodide, filtered through 1.2 ml Costar cluster tubes (Costar #4410), or 6 ml tubes with 35 μm strainer cap (Falcon 2235, Becton-Dickinson) in case of volumes greater than 0.5 ml.

  • 05% BSA) until use.

  • Briefly, to prepare AGE-BSA, essentially fatty-acid-free and endotoxin-free BSA (250 mg/ml) was incubated at 37 °C for 2, 5, and 10 wk with D-glucose (500 mM) in a 0.4-M phosphate buffer containing EDTA, ampicillin, Fungazone, polymixin B, and protease inhibitors.

  • Using these membranes, filtration binding assays were performed according to previously described protocols [ 28 ] . To reduce nonspecific binding, 96-well filtration plates (fiberglass FB filters; Millipore, Bedford, USA) were pre-coated as suggested by the manufacturer with 0.5% polyethyleneimine + 0.1% BSA and then washed four times with 0.1% BSA.

  • Cells were next washed with PBS and the fluorescence images of both oxidized OxyBURST Green H 2 HFF BSA and LysoTracker Red were recorded.


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