Example sentences for: brl

How can you use “brl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA from tomato leaves (5-15 mm), fruits (3-8 mm in diameter), whole flowers, and light-grown and dark-grown tomato seedlings was isolated with the Trizol Reagent from Gibco BRL (Gaithersburg, MD).

  • Dulbecco's Modified Eagle Medium (DMEM), hypoxanthine-aminopterin-thymidine (HAT) medium supplement, foetal calf serum (FCS) and gentamycin were purchased from Gibco BRL (Rockville, Maryland).

  • Ham's F12 medium, Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, type II collagenase, dispase, and Geneticin™ were supplied by Gibco-BRL (Rockville, MD, USA).

  • Culture medium was prepared with low-glucose Dulbecco's modified Eagle medium with 10mmol/l HEPES (GIBCO BRL, Grand Island, NY, USA), 0.1mmol/l nonessential amino acids (Sigma Chemical, St. Louis, MO, USA), 0.4 mmol/l L-proline (Sigma Chemical), and 1mmol/l gadolinium-DTPA (Magnevist, Berlex Imaging, Wayne, NJ, USA).

  • Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.


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