Example sentences for: brl

How can you use “brl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All cells were cultured at 37°C up to 10 5cells per ml in RPMI-1640 media, containing 10% Fetal Bovine Serum (FBS) treated with a mixture of 1% streptomycin and penicillin antibiotics, and 1% L-glutamine (Gibco/BRL).

  • A549 cells were grown to 50-80% confluence on 12-well plates and transfected with the Mercury pathway profiling system vector, pNFκB-TA-Luc (Clontech, Palo Alto, CA, USA), using lipofectamine (Gibco/BRL, Rockville, MD, USA) and used according to the manufacturer's recommendations.

  • HeLa cells were grown in DMEM (Gibco/BRL) supplemented with 10% fetal calf serum, seeded at 10 cells/well in 96-well microtiter plates and transfected 8 hours later using Exgen 500 (Euromedex, Souffelweyersheim, France).

  • Reverse transcription of RNA was performed using the Superscript II first strand synthesis system for RT-PCR (Gibco BRL), following the supplier's instructions.

  • The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).


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