Words similar to brl
Example sentences for: brl
How can you use “brl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Trizol reagent, deoxyribonucleaseI, and SuperScriptII reverse transcriptase were purchased from Gibco BRL (Rockville, MD).
Total RNA from tomato leaves (5-15 mm), fruits (3-8 mm in diameter), whole flowers, and light-grown and dark-grown tomato seedlings was isolated with the Trizol Reagent from Gibco BRL (Gaithersburg, MD).
The fusion proteins were transiently expressed in HEK 293 cells [ 37 ] . Transfections were done using Lipofectamine 2000 (Gibco BRL).
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
Two micrograms of total RNA were primed with oligo(dT) 15 and oligo(dN) 6 and were reverse transcribed into cDNA (Superscript II; Gibco/BRL) in a total volume of 20 μl.
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