Words similar to brl

Example sentences for: brl

How can you use “brl” in a sentence? Here are some example sentences to help you improve your vocabulary:

All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
All cells were cultured at 37°C up to 10 5cells per ml in RPMI-1640 media, containing 10% Fetal Bovine Serum (FBS) treated with a mixture of 1% streptomycin and penicillin antibiotics, and 1% L-glutamine (Gibco/BRL).
Total cellular RNA was extracted using the RNAzol reagent (Gibco/BRL).
Cells were then washed with Hank's buffered sterile saline solution and homogenized in 2 ml Triazol Reagent (Gibco/BRL), and RNA was purified in accordance with the manufacturer's instructions.
RNA was isolated from 50 mg of the joint powder with 1 ml Trizol reagent according to the manufacturer's instructions (Gibco BRL, Grand Island, NY, USA).