Example sentences for: brl

How can you use “brl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After all the components were assembled, 200 units of MMLV reverse transcriptase (Gibco BRL Life Technologies, Bethesda, MA) were added.

  • The 5'-end of the sagA/pel mRNA was determined by the extension of the 5'-end 32P-labeled oligonucleotides 5'-ACCTTATTTTAAAAATAAAGTTAA-3' following the method of Sambrook [ 45 ] . Oligonucleotides were labeled with [γ- 32P] ATP (10 mCi/mL in aqueous solution) (Amersham, Arlington Heights, IL) and T4 polynucleotide kinase (Gibco BRL Life Technologies, Rockville, MD).

  • The hybridization solution included 0.5 μg/ml poly(dA) (Research Genetics #POLYA.GF) and 0.5 μg/ml cot1 DNA (BRL #15279-011) as blocking agents.

  • Supernatants were recovered and incubated with protein-A agarose beads (Gibco BRL) coated with a rabbit anti-Syk antibody (SC-573, Santa Cruz Biotechnology, Inc.) for 30 min.

  • Reverse transcription of RNA was performed using the Superscript II first strand synthesis system for RT-PCR (Gibco BRL), following the supplier's instructions.


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