Example sentences for: brefeldin

How can you use “brefeldin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After 1-7 days, cells were stimulated for 4 h with 10 ng/ml PMA + 1 μg/ml ionomycin (both from Sigma), in the presence of 10 μg/ml Brefeldin A.

  • The cytokine analysis relies on the intracellular accumulation of cytokines in the Golgi compartment over the 15 hr. activation period induced by either brefeldin A or monensin.

  • Con A, phorbol myristate acetate (PMA), N-acetylcysteine (NAC), brefeldin A and monensin were obtained from Sigma (St.

  • 5 ng/ml) as described previously [ 25 ] . Either monensin (1 uM) or brefeldin A (1 ug/ml) was used in all cases to block Golgi function and allow cytokine accumulation with the cell.

  • For experiments examining proliferation, PBMC were incubated with SEB for 48 h at 37°C, with addition of 60 μM BrdU (Sigma) and 10 μg/ml Brefeldin A for the last 6 h.


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