Words similar to bpcr

Example sentences for: bpcr

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Figure 2shows the map and sequence of templates and primer sites used in BPCR between a portion of plasmid pBS and Phagescript, a derivative of the ssDNA M13 phage.
Each of the two samples was serially diluted into BPCR reactions, the dilution involving only the cut and intact template, with everything else unchanged.
Several variations of BPCR with Taq polymerase were carried out (data not shown), including: 1) cut and intact templates were prepared with primers b1 and b2, 2) purified ssDNA instead of phage particles was used as a reference template and 3) cut and intact templates were prepared with the Stoffel fragment of Taq polymerase.
PCR itself generates new 3'ends [ 2 3 ] . In BPCR new ends yield recombinant products, even if the initial template was completely intact.
Immediately following pre-extension, i.e. without storage at 4°C, the samples were serially diluted and PCR amplified with the BPCR primers.