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In the examination of the discordance between human and pig results, Bowkers test for symmetry using categorized data was statistically significant (p < 0.001) indicating that, in general, stronger hybridisation signals were detected from human than from pig.
The kappa will have lower values than other correlation coefficients when the marginal distributions are unequal, and thus may represent a more realistic assessment of the underlying agreement [ 7 ] . Bowkers test for symmetry [ 14 ] was used to test the hypothesis that detectable expression from pig and human did not differ systematically.