Words similar to bovine
Example sentences for: bovine
How can you use “bovine” in a sentence? Here are some example sentences to help you improve your vocabulary:
Medulloblastoma DAOY cells were obtained from the American Type Culture Collection and maintained in Improved MEM Zn ++Option (Richter's Modification) medium containing supplemented with 20% fetal bovine serum (Life Technologies, Inc., USA).
4 for MRLC), blotted to PVDF or nitrocellulose membranes, immunostained with our rabbit polyclonal anti-HSP20 antibody (1:5000) or rabbit polyclonal anti-MRLC antibody (1:4000 in 1% bovine serum albumin and 0.01% sodium azide), and detected with enhanced chemifluorescence (ECF) for HSP20 and enhanced chemiluminescence (ECL) for MRLC [ 22 ] . A representative blot is shown in Fig.
They were routinely grown in a monolayer (30%-50% confluence) on a glass cover slide at 37°C in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 1% L -glutamine, 1% HEPES [4-(2-hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)], 0.5% gentamicin, 1% penicillin/streptomycin and 10% Fetal Bovine Serum (Gibco, NY).
Cells were grown and maintained in Dulbecco's Modified Eagle Medium (Life Technologies) that was supplemented with 10% fetal bovine serum (Hyclone), 170 mM glutamine and penicillin/streptomycin (100 μg/ml, Life Technologies) in humidified air containing 5% carbon dioxide at 37°C.
Purified bovine CL-derived microvascular endothelial cell populations were generated commercially by Cambrex Biosciences (BioWhittaker, Walkersville, MD) as described in detail [ 35 ] . In the present study, CLENDO cells from frozen aliquots (passage 3) were seeded (5,000 cells/cm 2) in 6- or 24-well plates or 10 cm culture dishes.
Loading...