Words similar to bovine
Example sentences for: bovine
How can you use “bovine” in a sentence? Here are some example sentences to help you improve your vocabulary:
The digestion step was repeated twice, and the 3 cell isolates were pooled in mouse osteoblast growth medium (OBGM), consisting of Dulbecco's modified Eagle's medium containing 25 mM HEPES, 10% fetal bovine serum (Sigma), 2 g of sodium bicarbonate per liter, 75 μg of glycine/ml, 100 μg of ascorbic acid/ml, 40 ng of vitamin B 12 /ml, 2 μg of p -aminobenzoic acid/ml, 200 ng of biotin/ml, and penicillin (100 U/ml)-streptomycin (100 μg/ml)-amphotericin B (25 μg/ml) (pH 7.4) [ 60 ] . Cells were seeded in six-well cluster plates and incubated at 37°C in a 5% CO 2 atmosphere until they reached confluence (6 to 7 days).
This alignment could be extended to the enzymatic core of three characteristic Mg 2+-dependent endonucleases: the bovine DNAseI [ 29 ] and two apurinic/apyrimidinic (AP) DNA-repair endonucleases, i.e. the E. coli Exonuclease III [ 30 ] and the human HAP1 protein [ 31 ] . These nucleases share the same catalytic residues and form a similar four-layered α/β-sandwich motif.
RPMI-1640 medium without phenol red, cell culture freezing medium containing DMSO, fetal bovine serum (FBS), penicillin, streptomycin, 7.5% bovine serum albumin (BSA), phosphate buffer saline (PBS, pH = 7.4), and trypan blue were purchased from Life Technologies (Grand Island, NY).
In a subset of experiments, GC were cultured in a defined serum-free medium of HEPES-buffered DMEM:F10 (1:1) containing insulin (300 mU/ml), hydrocortisone (40 ng/ml), transferrin (5 μg/ml), bovine serum albumin (4 mg/ml, Bovine Albumin Fraction V, albumin = 95%),
RPMI 1640 media, fetal bovine serum, and GlutaMAX™-I were obtained from Life Technologies (Rockville, MD).
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