Example sentences for: bovine

How can you use “bovine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A human lung adenocarcinoma cell line, NCI-H441, was maintained in vitro in monolayer culture in 10% fetal bovine serum in the presence of penicillin (100 U/ml), streptomycin (100 μg/ml) and fungizone (0.

  • Stress proteins have been shown to be expressed in non-stressed cells during development and differentiation [ 25 ] . Hsps were shown to be expressed during the differentiation of mammalian osteoblasts and promelocytic leukemia cells [ 26 ] . In addition, hsp expression has been shown to accompany growth arrest in human B lymphocytes [ 27 ] and macrophage differentiation of HL 60 cells [ 28 ] . During myogenic differentiation, mRNA for alphaB increases in conjunction with the induction of mRNA for myogenin, the earliest known event in myogenesis [ 29 ] . The addition of exogenous alpha-crystallin to primary bovine lens epithelium was shown to induce rapid changes in cell shape, leading to the formation of lentoid bodies [ 21 ] . These studies strongly suggest that the hsp family of proteins has other functions in addition to protecting proteins and cells during stress.

  • 5 μg total protein) were incubated in polypropylene tubes with 2 nM [ 3H]-Hh-Ag 1.5 in the presence or absence of 2 μM competitor in binding buffer (50 mM Tris pH 7.5, 5 mM MgCl, 1 mM EDTA, 0.1 % bovine serum albumin) plus EDTA-free protease inhibitor cocktail (Roche) in a final volume of 250 μl for 1-26 hours at 37°C.

  • ARPE-19 cells [ 25 ] were obtained from the American Type Culture Collection and grown in DMEM/F12 containing 10% heat-inactivated fetal bovine serum and 2.5 mM glutamine.

  • Human embryonal kidney (HEK) cells (line 293) and NIH 3T3 mouse fibroblasts were obtained from American Type Culture Collection (Rockville, MD) and were maintained at 37°C in a 5% CO 2 atmosphere in Dulbecco's modified Eagle's medium containing 10% (v/v) fetal bovine serum.


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