Words similar to bou

Example sentences for: bou

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The SIM4 tool [ 18] was then applied to map the exon-intron splicing coordinates of individual BOU and EST sequences more precisely.
For each EST library, we then calculated the fraction of member ESTs that were deposited in the same orientation as the BOU sequence of the UniGene cluster to which they belonged.
Our goal of calculating library quality by estimating the 'correctness' of EST orientation is complicated by the fact that not every UniGene cluster contains an mRNA with a defined open reading frame (ORF), and not every BOU sequence is deposited in the correct orientation (in other words, the correct orientation of many UniGene clusters is not known definitively).
To assess the quality of directional cloning in EST libraries, we applied the MEGABLAST tool [ 17] to align ESTs to the BOU of the UniGene cluster to which they belonged.
This analysis was sufficient for UniGene clusters with an oriented BOU (see above).