Words similar to borate
Example sentences for: borate
How can you use “borate” in a sentence? Here are some example sentences to help you improve your vocabulary:
The 32P end-labeled oligonucleide probe (5 nM) was incubated with immunoprecipitation-purified FLAG-rDEK in a range of concentrations from one tenth to 10 times the estimated K d[app] (apparent dissociation constant) as described elsewhere [ 31 ] . Protein-bound DNA was separated from free probe as for EMSA in 1 × TBE (Tris-borate-EDTA buffer: Tris 89 mM, borate 89 mM, EDTA 2 mM).
BAL = bronchoalveolar lavage; BSA = bovine serum albumin; C/EBP = CCAAT/enhancer binding protein; COX-2 = cyclooxygenase-2; CREB = cAMP response element binding protein; FBS = fetal bovine serum; fMLP = n-formyl-methionyl-leucyl-phenylalanine; HBSS = Hank's balanced salt solution; ICAM-1 = intercellular adhesion molecule-1; LPS = lipoplysaccharide; MAP = mitogen activated protein; MIP-2 = macrophage inflammatory protein-2; NF-κB = nuclear factor-kappa B; PBS = phosphate-buffered saline; PI3K = phosphatidyl inositol 3 kinase; PKB = protein kinase B; RT-PCR = Reverse transcription-polymerase chain reaction; TBE = Tris borate EDTA; TNF-α = tumor necrosis factor alpha
0% acrylamide, 7.7 M urea, 89 mM Tris, 89 mM borate, 2.5 mM EDTA, pH 8.3).
The E-TP binary complexes formed were resolved at 4°C on 6% native polyacrylamide gel using Tris-Borate buffer (85 mM Tris, 85 mM Boric acid, pH 8.0).
The cells were incubated in 0.1 M borate buffer (pH 8.5, 0.1 M boric acid, 25 mM Na 2 B 4 O 7 , and 75 mM NaCl) twice for 5 minutes each (10 minutes total), followed by three 10 minute washes in PBS.