Words similar to boiling

Example sentences for: boiling

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To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
Plasmid DNA was isolated by the boiling lysis method [ 60 ] , followed by precipitation with polyethylene glycol 8000.
Samples were incubated in boiling water for 5 minutes then passed through a 20-gauge and 26-gauge needle repeatedly to shear chromosomal DNA.
This proteoglycan resisted elution by 8 M urea but was eluted by boiling the Sepharose beads in SDS (Fig.
The hybridization procedure was performed as described before [ 42 ] . Several duplicate membranes were prepared and the radioactive probe was stripped after each round of hybridization by boiling for 10 minutes in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1% SDS.