Example sentences for: boiled

How can you use “boiled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For re-precipitation, the pellet was boiled in 10 μl 0.1% SDS for 5 min and diluted 1:20 in the Triton X lysis buffer followed by the precipitation procedure.

  • Among those that consumed tap water at home sometimes or always (47/49 [96%] of cases and 68/99 [69%] of controls), 20/47 (42%) of cases and 32/68 (47%) of controls filtered their water sometimes or always, and 5/47 (11%) of cases and 10/68 (15%%) of controls boiled their water sometimes or always.

  • The homogenate then was boiled for 5 min at 100°C and debris was removed by centrifugation for 10 min at 15000 rpm.

  • Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.

  • Matched samples from the proteolysis reaction were quenched with trichloroacetic acid (10%, w/v), protein was sedimented, then boiled in (75 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% 2-mercaptoethanol and 0.01% bromophenol blue), subjected to SDS-PAGE using 10 or 15% resolving gels, and protein bands visualized by Coomassie staining.


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