Words similar to boiled
Example sentences for: boiled
How can you use “boiled” in a sentence? Here are some example sentences to help you improve your vocabulary:
The situation, at least today, boiled down to too much demand chasing too little supply.
After the digestion the samples were boiled for 10 min to inactivate the enzyme.
SDS-PAGE sample buffer containing 10 mM DTT was added to the washed beads, and the samples were heated at 65°C for 1 min ( 3H fatty acid labeled samples) or boiled for 5 min ( 35S methionine/cysteine labeled samples).
For Western blot analysis, 15 μl of samples containing 20 μg protein were mixed with equal volume of 2 × loading buffer (1X = 125 mM TRIS-HCl, pH 6.8; 2% SDS, 5% glycerol, 0.003% bromphenol blue and 1% 1-mercaptoethanol), and boiled for 5 min.
Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.
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