Words similar to ble

Example sentences for: ble

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2) The ble gene was recovered as a 382-bp Bam HI fragment from pT7Blue-bleA and cloned into Bam HI cleaved pMV261 to generate pMV261-ble.
Cells were grown to approximately 75% confluence and incubated in the presence of 8 μg/ml polybrene with 2 ml supernatant containing amphotropic retrovirus DFG-IRAP-zeo rcontaining the human IL-1Ra and Streptoalloteichus hindustanus bleomycin-resistance ( Sh ble ; zeo r) genes.
2 plasmid contains an oriE for plasmid replication in E. coli , the mycobacteriophage L5 integrase and attP site to allow integration into the M. smegmatis chromosome, two selectable genes, ble (bleomycin or zeocin resistance) and amp (ampicillin resistance), and the M. tuberculosis RV1221 ORF cloned downstream of the M. tuberculosis hsp60 promoter.
1) The ble gene was PCR amplified from pPICZalphaC as a 375-bp fragment using primers Ble-N and Ble-C; the amplicon was blunt-ended and ligated into the blunt-cloning site of pT7Blue to generate pT7Blue-bleA.