Words similar to biovar
Example sentences for: biovar
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Historically, it has been very difficult to irrefutably trace natural Brucella transmission unless the strain involved happened to belong to a rare biovar.
The bacterial strains used for this study included the complete array of Brucella type strains and/or FAO/WHO Reference Strains: B. abortus biovar 1, strain 544 (ATCC-23448); B. abortus biovar 1, strain S19 (isolates from stock culture and from infected cattle); B. abortus biovar 1, strain 2308; B. abortus biovar 1, strain RB51 (from the NADC Culture Collection and from commercially prepared vaccine production lots); B. abortus biovar 2, strain 86/8/59, (ATCC-23449); B. abortus biovar 3, strain Tulya, (ATCC-23450); B. abortus biovar 4, strain 292, (ATCC-23451); B. abortus biovar 5, strain B3196, (ATCC-23452); B. abortus biovar 6, strain 870, (ATCC-23453); B. abortus biovar 9, strain C68, (ATCC-23455); B. canis , RM-6/66, strain (ATCC-23365); B. melitensis biovar 1, strain 16M, (ATCC-23456); B. melitensis biovar 2, strain 63/9, (ATCC-23457); B. melitensis biovar 3, strain Ether, (ATCC-23458); B. neotomae , strain 5K33, (ATCC-23459); B. ovis , strain 63/290, (ATCC-25840); B. suis biovar 1, strain 1330, (ATCC-23444); B. suis biovar 2, strain Thomsen, (ATCC-23445); B. suis biovar 3, strain 686, (ATCC-23446); B. suis biovar 4, strain 40, (ATCC-23447); B. suis biovar 5, strain 513, (ATCC-NA).
A small panel of B. abortus biovar 1-field strains, consisting of 17 bovine isolates, 2 elk isolates and 3 bison isolates, was tested to look at strain diversity in the U.S.
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
There was disagreement between the fragment sizes calculated from the published genomic sequences and the observed fragment sizes amplified from some of the VNTR loci in B. melitensis biovar 1 and B. suis biovar 1 (e.g.
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