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Example sentences for: bifunctional
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Examples are the bifunctional proteins ThrA (aspartokinase I and homoserine dehydrogenase I) and MetL (aspartokinase II and homoserine dehydrogenase II) where only the amino-terminal modules representing the kinase activities have been identified on the basis of their sequence similarity to the E. coli unimodular aspartokinase III (LysC).
In C. elegans , two genes each encode unusual bifunctional enzymes containing both isocitrate lyase and malate synthase domains [ 49 ] . Since the isocitrate lyase domain lies within the amino-terminal half of the C. elegans bifunctional enzyme and none of the Meloidogyne EST reads stretches across both domains, further sequencing of the 3' end of cDNA clones from the M. hapla or M. javanica isocitrate lyase ESTs will be necessary to determine whether the Meloidogyne genus contains a bifunctional glyoxylate enzyme homolog similar to that of
When two paralogs of such a bifunctional gene are produced by duplication, each may be driven by positive selection to specialize in one of these functions, which it performs more efficiently than the ancestor gene, leading to the creation of two indispensable genes with distinct functions [ 11].
Rabbit anti-PKA RIα was prepared by synthesizing the N-terminal 18 residues of human RIα protein and conjugating this peptide (MESGSTAASEEARSLREC) to keyhole limpet haemocyanin (KLH) via the bifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MES).
GSPS is a bifunctional enzyme: its synthase domain carries out the ATP-dependent fusion of two glutathione moieties to the polyamine spermidine to form the antioxidant metabolite GSP, while its amidase domain hydrolyzes the amide linkage to release glutathione and spermidine [ 26, 55, 56].
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