Example sentences for: bicistronic

How can you use “bicistronic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • JC constructed and characterized the bicistronic reporter constructs and aided with manuscript preparation.

  • The effect of constitutively active 4EBP-1 on the activity of the p27 Kip15'-UTR was determined by cotransfecting the bicistronic construct together with an expression vector encoding 4EBP-1-5A into MCF7 cells.

  • When placed between the CAT and luciferase coding regions in a bicistronic mRNA, the 5'-UTR stimulates high level expression of the downstream coding region (luciferase).

  • To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.

  • This property is shared by the highly homologous mouse p27 Kip15'-UTR [ 28 ] . Coexpression of 4EBP-1-5A with the bicistronic construct containing the p27 Kip15'-UTR leads to an approximately two-fold increase in cap-independent expression.


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