Words similar to bicistronic
Example sentences for: bicistronic
How can you use “bicistronic” in a sentence? Here are some example sentences to help you improve your vocabulary:
To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.
The effect of constitutively active 4EBP-1 on the activity of the p27 Kip15'-UTR was determined by cotransfecting the bicistronic construct together with an expression vector encoding 4EBP-1-5A into MCF7 cells.
When placed between the CAT and luciferase coding regions in a bicistronic mRNA, the 5'-UTR stimulates high level expression of the downstream coding region (luciferase).
JC constructed and characterized the bicistronic reporter constructs and aided with manuscript preparation.
This property is shared by the highly homologous mouse p27 Kip15'-UTR [ 28 ] . Coexpression of 4EBP-1-5A with the bicistronic construct containing the p27 Kip15'-UTR leads to an approximately two-fold increase in cap-independent expression.