Words similar to bicistronic
Example sentences for: bicistronic
How can you use “bicistronic” in a sentence? Here are some example sentences to help you improve your vocabulary:
JC constructed and characterized the bicistronic reporter constructs and aided with manuscript preparation.
The effect of constitutively active 4EBP-1 on the activity of the p27 Kip15'-UTR was determined by cotransfecting the bicistronic construct together with an expression vector encoding 4EBP-1-5A into MCF7 cells.
When placed between the CAT and luciferase coding regions in a bicistronic mRNA, the 5'-UTR stimulates high level expression of the downstream coding region (luciferase).
To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.
This property is shared by the highly homologous mouse p27 Kip15'-UTR [ 28 ] . Coexpression of 4EBP-1-5A with the bicistronic construct containing the p27 Kip15'-UTR leads to an approximately two-fold increase in cap-independent expression.