Words similar to bethesda
Example sentences for: bethesda
How can you use “bethesda” in a sentence? Here are some example sentences to help you improve your vocabulary:
All enzyme reactions were performed as recommended by the manufacturers (GIBCO Bethesda Research Laboratories, Inc., Gaithersburg, Md., New England Biolabs, Beverly, Mass.)
Renbaum Bethesda, Md.
PCR was performed using a standard protocol as follows with a final volume of 50 μl; 5 μl 10X PCR buffer, 5 μl 2 mM dNTP, 5 μl forward primer (concentration 20 ng/ul), 5 ul reverse primer (concentration 20 ng/ul), 1.5 μl 50 mM MgCl, 0.25 μl Taq polymerase (5 U/μl), and 1 μl sample purified genomic DNA (concentration approximately 30 ng/μl) (PCR reagents, Gibco-BRL, Bethesda, MD).
The video images were captured by CG-7 color frame grabber (Scion Corporation, Frederick, MD) supported by Scion Image public software developed at the National Institutes of Health (Wayne Rasband, NIH, Bethesda, MD), and ported to Windows XP, 2002 release (Microsoft Corporation, Redmont, WA).
The non-redundant (NR) database of protein sequences (National Center for Biotechnology Information, NIH, Bethesda) was searched using the BLASTP program [ 100 ] . Position-specific scoring matrix (PSSM) searches were conducted using the PSI-BLAST program, typically with a PSSM inclusion expectation (E) value threshold of 0.01, and were iterated until convergence [ 100 101 ] . Prior to PSI-BLAST searches, query sequences were evaluated for compositional bias using the SEG program [ 102 ] . If no such bias was detected, searches were run with the composition-based statistics turned off, in order to maximize sensitivity [ 103 ] . Multiple alignments of protein sequence were constructed using the T_Coffee program [ 104 ] , followed by manual correction based on the PSI-BLAST results.
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