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Example sentences for: bellefonte

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Samples of 20 to 50 μL were injected onto a Discovery Amide C16 or Discovery C18 reverse phase column (15 cm × 4.6 mm, 5 μm, Supelco, Bellefonte, PA), and analytes were separated using the ion-pair chromatographic method.
Fatty acid methyl ester levels were measured by gas chromatography using a previously described program [ 69 ] . Two μl aliquots of each fatty acid methyl ester extract were injected onto a 5890 Series II gas chromatograph from Hewlett Packard containing a 30 m SP2330 column with an inner diameter of 0.75 mm (Supelco, Bellefonte, PA, USA).
The seeds/seedlings were incubated in 0.6 to 2.0 ml 1 N methanolic-HCl (Supelco, Bellefonte, PA, USA) at 80 to 83°C for 1 to 2 h.
Fatty acids were identified by comparison of their retention times to those of fatty acid standards (Supelco, Inc., Bellefonte, PA) and results were expressed as weight percent of total fatty acids.
Cholesterol was analyzed using a Hewlett-Packard gas liquid chromatograph (model 5890) equipped with a flame ionization detector and a fused-silica capillary column (SAC-5, 30 m × 0.25 mm, Supelco, Bellefonte, PA) and helium carrier gas.