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Example sentences for: beckton

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The samples were filtered through 35 μm cell strainer caps of Falcon polystyrene tubes before being applied to a Beckton Dickinson Fascan equipped with CellQuest software version 3.2.
The CD4 +CD25 +-expressing T cells were identified by staining spleen cells with fluorescein isothiocyanate (FITC)-labeled anti-CD4 and biotin-labeled anti-CD25 followed by CyChrome-labeled streptavidin (BD PharMingen, San Diego, CA, USA), and analyzed on a flow cytometer (Beckton Dickinson, San Jose, CA, USA).
Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).
The suspended cells were washed with PBS, resuspended in growth medium, and plated on FCS-coated glass coverslips for microscopy, or onto 35-mm or 100-mm Falcon plastic culture dishes (Beckton Dickinson, Lincoln, NJ) or FCS-coated glass culture dishes for biochemical experiments.