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Example sentences for: beckton

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The CD4 +CD25 +-expressing T cells were identified by staining spleen cells with fluorescein isothiocyanate (FITC)-labeled anti-CD4 and biotin-labeled anti-CD25 followed by CyChrome-labeled streptavidin (BD PharMingen, San Diego, CA, USA), and analyzed on a flow cytometer (Beckton Dickinson, San Jose, CA, USA).
The samples were filtered through 35 μm cell strainer caps of Falcon polystyrene tubes before being applied to a Beckton Dickinson Fascan equipped with CellQuest software version 3.2.
The suspended cells were washed with PBS, resuspended in growth medium, and plated on FCS-coated glass coverslips for microscopy, or onto 35-mm or 100-mm Falcon plastic culture dishes (Beckton Dickinson, Lincoln, NJ) or FCS-coated glass culture dishes for biochemical experiments.
Cells were then labeled with phycoerythrin-conjugated anti-CD11b antibodies (Pharmingen) or fluorescein-conjugated RANKL (a gift from M Tondravi, American Red Cross, Rockville, MD, USA) as described previously [ 33 ] . Data were acquired using a FACScalibur instrument (Beckton Dickenson, Bedford, MA, USA) and were analyzed by Cellquest software (version 3.1, Beckton Dickenson).