Words similar to beads
Example sentences for: beads
How can you use “beads” in a sentence? Here are some example sentences to help you improve your vocabulary:
To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.
The supernatant was gently mixed with glutathione-sepharose beads (Amersham-Pharmacia) at 4°C for 30 min.
About 20 μl of the beads slurry was transferred to a 1.5 ml tube, then washed 4 times with 300 μl bead washing buffer (1 × TE + 100 mM NaCl).
To this end, 35S-labelled E2F was produced in vitro in a wheat germ lysate and incubated with the WD repeat region of Slimb fused to GST and bound to beads.
Enzymes were purified from fermentation broths utilizing the BioCAD 700E system (Perceptive Biosystems, Foster City, CA) using POROS HS/M resin (Perceptive Biosystems, Foster City, CA) on porous polystyrene beads, and was stored as stock solutions at 20 mg/ml in 20 mM MES, pH 5.5 with 1 mM CaCL 2 at -20°C.