Example sentences for: beads

How can you use “beads” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.

  • The supernatant was gently mixed with glutathione-sepharose beads (Amersham-Pharmacia) at 4°C for 30 min.

  • About 20 μl of the beads slurry was transferred to a 1.5 ml tube, then washed 4 times with 300 μl bead washing buffer (1 × TE + 100 mM NaCl).

  • To this end, 35S-labelled E2F was produced in vitro in a wheat germ lysate and incubated with the WD repeat region of Slimb fused to GST and bound to beads.

  • Enzymes were purified from fermentation broths utilizing the BioCAD 700E system (Perceptive Biosystems, Foster City, CA) using POROS HS/M resin (Perceptive Biosystems, Foster City, CA) on porous polystyrene beads, and was stored as stock solutions at 20 mg/ml in 20 mM MES, pH 5.5 with 1 mM CaCL 2 at -20°C.


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