Words similar to baculovirus
Example sentences for: baculovirus
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Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
The antibody serum also reduced Cry1Aa activity against B. mori when it was fed to larvae prior to toxin addition to the diet [ 14 ] . The same group cloned and introduced the BtR175 gene with a baculovirus vector into Spodptera frugiperda Sf9 cells.
In contrast, another group of entomopoxvirus proteins, such as MSV226 from MSV and AMV262 from AMV, showed similarity only to the amino-terminal part of the baculovirus Bro proteins.
To investigate this relationship further, we performed BLAST searches of the database with all members of the baculovirus LD130 family and also with all sequences that showed alignments with E values of less than 10 -3to members of this family.
In contrast to the close relatedness of baculovirus gp64 homologs, the LD130 homologs are highly variable suggesting that gp64 was recently incorporated into a baculovirus genome where it displaced the envelope fusion function of the ld130 homologs [ 3].