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Example sentences for: bacs
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BACs were isolated from RPCI-11 male and RPCI-13 female BAC libraries as described [ 23, 24].
To mark the regions of low-copy repeats in human 15q11-q13, we utilized a probe derived from the donor locus of a major component of the PWS/AS breakpoint duplication clusters, PAC clone pDJ-778A2 which contains a significant portion of the HERC2 gene in 15q13 [ 33 ] . In addition, we selected probes corresponding to sites distal to the PWS/AS domain flanking a large inverted-duplicated 15 (inv dup(15)) supernumerary marker chromosome [ 23 ] . Specifically, probe RPCI-11-88O16 (abbreviated RP11-88O16) was selected for FISH analysis because it lies distal to the inversion-duplication event breakpoint and is linked to STS marker D15S1010 [ 23 24 ] . Upon reaching the PTR inversion breakpoint interval with probe RP11-40J8, flanking BACs were chosen from the November 2002 human genome assembly to establish the extent of the interval and define the boundaries of the interval.
The BACs at the boundaries between euchromatin and centric heterochromatin (see Table 1) identify 2.1 Mb (150 curated gene models) of heterochromatic sequence at the centric ends of the high-quality Release 3 sequence spanning the euchromatin [ 47].
A BAC-based physical map of the X chromosome was constructed by the European Drosophila Genome Project (EDGP) [ 12] and used as a starting point for BAC-based sequence assembly of the X. In our finishing work, we replaced mismapped BACs at 13A and 14D.
We have also identified BACs containing the rolled, PARP and SNAP25 genes in pilot STS content mapping experiments in the heterochromatin.
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