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Example sentences for: background-subtracted

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Thus, normalization of background-subtracted images of the same array for different hybridizations was accomplished essentially by multiplying all pixels in the non-reference image by a constant scale-factor, the value of which had been estimated by SVD.
In the final step, this integrated background value is subtracted from the raw integrated spot intensity to produce the background-subtracted integrated intensities we use for further analysis.
1are the background-subtracted images of the array after hybridization with end-labeled oligo(dT), performed in order to measure accessible PolyA in the spots.
5, with the location, amplitude, and standard deviation coefficients all estimated automatically from the background-subtracted image in two dimensions, using the Levenberg-Marquardt method [ 10 ] . The similarity between background-subtracted and fitted spot images (Fig.
Therefore, in order to be able to compare background-subtracted images of the same 12 × 30 spot array for successive hybridizations, normalization was performed as follows.