Example sentences for: azide

How can you use “azide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 4 for MRLC), blotted to PVDF or nitrocellulose membranes, immunostained with our rabbit polyclonal anti-HSP20 antibody (1:5000) or rabbit polyclonal anti-MRLC antibody (1:4000 in 1% bovine serum albumin and 0.01% sodium azide), and detected with enhanced chemifluorescence (ECF) for HSP20 and enhanced chemiluminescence (ECL) for MRLC [ 22 ] . A representative blot is shown in Fig.

  • After 3 washes with phosphate-buffered saline (PBS) and 0.05% Tween 20 (Sigma), plates were blocked with PBS plus 2% BSA (Sigma), and 0.02% sodium azide (Sigma), for 2 hours at room temperature (RT).

  • Detached cells were washed twice with staining buffer (PBS containing 10% FCS, 0.1% sodium azide and 25 mM HEPES, pH 7.4) and stained as described previously [ 49 ] . Briefly, cells were transferred into the wells of round-bottom 96-well plates, the plates were centrifuged at 200 g for 30 seconds, the supernatant removed by shaking, and the cell pellets resuspended in 50 μl of staining buffer containing saturating concentration of anti-MHCII-FITC.

  • Cell–cell conjugates were disrupted by washing the cells with PBS supplemented with 0.02% azide and 0.5 mM EDTA, and mixed vigorously, then stained with additional Abs.

  • 02% sodium azide blocking solution and allowed to sit overnight at 4°C.


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