Words similar to azide
Example sentences for: azide
How can you use “azide” in a sentence? Here are some example sentences to help you improve your vocabulary:
Endogenous peroxidase activity was blocked with 0.1 mol sodium azide containing 1% hydrogen peroxide, and the specimens were incubated with 10% normal goat serum, 2% normal rat serum, and 1% bovine serum albumin for 30 min at room temperature to eliminate non-specific binding.
The incubation was ended by addition of an equal volume of 20 mM sodium azide.
Detached cells were washed twice with staining buffer (PBS containing 10% FCS, 0.1% sodium azide and 25 mM HEPES, pH 7.4) and stained as described previously [ 49 ] . Briefly, cells were transferred into the wells of round-bottom 96-well plates, the plates were centrifuged at 200 g for 30 seconds, the supernatant removed by shaking, and the cell pellets resuspended in 50 μl of staining buffer containing saturating concentration of anti-MHCII-FITC.
02% sodium azide blocking solution and allowed to sit overnight at 4°C.
After 3 washes with phosphate-buffered saline (PBS) and 0.05% Tween 20 (Sigma), plates were blocked with PBS plus 2% BSA (Sigma), and 0.02% sodium azide (Sigma), for 2 hours at room temperature (RT).