Words similar to azide
Example sentences for: azide
How can you use “azide” in a sentence? Here are some example sentences to help you improve your vocabulary:
Detached cells were washed twice with staining buffer (PBS containing 10% FCS, 0.1% sodium azide and 25 mM HEPES, pH 7.4) and stained as described previously [ 49 ] . Briefly, cells were transferred into the wells of round-bottom 96-well plates, the plates were centrifuged at 200 g for 30 seconds, the supernatant removed by shaking, and the cell pellets resuspended in 50 μl of staining buffer containing saturating concentration of anti-MHCII-FITC.
Endogenous peroxidase activity was blocked with 0.1 mol sodium azide containing 1% hydrogen peroxide, and the specimens were incubated with 10% normal goat serum, 2% normal rat serum, and 1% bovine serum albumin for 30 min at room temperature to eliminate non-specific binding.
The incubation was ended by addition of an equal volume of 20 mM sodium azide.
After centrifugation, the cell pellet was resuspended with staining buffer (prechilled to 4°C) consisting of phosphate-buffered saline solution with 0.2% BSA and 1 μg/ml sodium azide.
At the indicated times cells were harvested, washed twice with phosphate-buffered saline (containing 0.1% serum bovine albumin and 0.1% sodium azide), and incubated for 30 min at 4°C with specific monoclonal antibodies to either CD3, CD4 or CD8.
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