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Example sentences for: awo

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Total genomic DNA from B. thuringiensis strain AWO6 hybridized with 23 pXO2 ORF fragments, including all ORFs tested in the region between ORF 5 and ORF 38 (Table 3).
Total DNA extracted from B. thuringiensis AWO6 was used to synthesize probe by incorporating [α- 32P]dCTP (6000 μCi/mMol) (NEN, Boston, MA) into randomly primed DNA synthesis reactions using the Megaprime DNA Labeling System (Amersham-Pharamacia Biotech, Piscataway, NJ) according to the manufacturer instructions.
The pXO2 ORF sequences detected in B. thuringiensis 33679 and B. thuringiensis AWO6 were distributed across the entire plasmid sequence, except in the 25.
The membrane containing B. thuringiensis AWO6 DNA was hybridized using a [α- 32P]dCTP-labeled probe prepared from a mixture of six PCR-amplified pXO2 ORF fragments (pXO2 ORFs 6, 10, 50, 63, 72, 81).
The two isolates with the most sequence conservation with pXO2 ORFs, B. thuringiensis isolates 33679 and AWO6, are known to contain large plasmids [ 12 19 ] . Four ORFs with high sequence similarity to B. thuringiensis AWO6 plasmid pAW63 were detected [ 22 ] , and a mixed pXO2 ORF probe hybridized with a PFGE fragment similar in size to pAW63.