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The two isolates with the most sequence conservation with pXO2 ORFs, B. thuringiensis isolates 33679 and AWO6, are known to contain large plasmids [ 12 19 ] . Four ORFs with high sequence similarity to B. thuringiensis AWO6 plasmid pAW63 were detected [ 22 ] , and a mixed pXO2 ORF probe hybridized with a PFGE fragment similar in size to pAW63.
B. thuringiensis strain AWO6 produced the most products in the PCR assay.
B. thuringiensis AWO6 is a strain containing a 70 kb plasmid designated pAW63 [ 12 21 ] . This strain was derived from B. thuringiensis HD73 by curing of its crystal toxin bearing plasmid, pHT73 [ 12 21 ] . The pAW63 plasmid contains a replication complex that is classified as a member of the pAMB-1 family of theta replicating plasmids that are present in a broad range of Gram positive species [ 22 ] . Plasmid pXO2 also appears to be a pAMB1-like theta replicating plasmid [ 23 ] and elements surrounding the replication complex are present in both pXO2 and pAW63 (see pXO2 ORFs 35, 37, 38, 39 in Tables 2and 3).
Total genomic DNA from B. thuringiensis strain AWO6 hybridized with 23 pXO2 ORF fragments, including all ORFs tested in the region between ORF 5 and ORF 38 (Table 3).
3 kb pXO2 cap gene-containing region did not hybridize with B. thuringiensis strain AWO6 DNA.