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Example sentences for: atpase
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The subunit A protein is approximately 70 kDa in most organisms studied and is a hydrophilic peptide located in the head group of the V 1 peripheral sector in three copies per holoenzyme [ 18 ] . This subunit contains a nucleotide binding motif and functions to bind and hydrolyze ATP [ 19 ] . In addition, subunit A contains a highly conserved cysteine residue located within the enzymes' catalytic center that may be involved in regulation of the holoenzyme [ 20 21 22 23 ] . Cloning, sequencing and characteristics of subunit A of the V-Type ATPase from Arabidopsis thaliana have been previously reported [ 24 ] .
Proton pumping ATPases/ATPsynthases are found in all groups of present day organisms [ 22 ] . The typical archaeal ATPsynthase is homologous to the eukaryotic vacuolar ATPase.
ATPase 6 is a component of the F 0 F 1 ATP synthase complex, which converts the energy stored in the form of the proton motive force to chemical energy in the form of ATP.
It has been reported before that an increased excretion of orotate in sick children may not be uncommon and that a positive allopurinol load test may indicate unrecognized mitochondrial disease, making orotic acid as a potential marker of mitochondrial dysfunction [ 35 ] . A potential link between mitochondrial cytopathies and urea cycle dysfunction has also been suggested by the findings of hypocitrullinemia in patients with neurogenic weakness, ataxia and retinitis pigmentosa (NARP) syndrome (Mendelian Inheritance in Man database *551500) caused by a T8993G mutation in mtDNA that triggers ATPase deficiency [ 36 37 ] . It was hypothesized that the NARP mutation would cause complex V deficiency in the small intestine as well, thus reducing the availability of mitochondrial ATP required for citrulline synthesis.
When E. coli Bl21(DE3), a strain that did not express additional rare tRNAs, was transformed with the cloned T. acidophilum ATPase A subunit, no additional protein bands were observed in extracts of induced cells (not shown).
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