Example sentences for: atp

How can you use “atp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 6panels C, E) supports the conclusion that phorbol esters decrease K ATP sensitivity to ATP.

  • Organic phosphate compounds are the central metabolites of all biological systems [ 1 2 ] . Some are the basic building blocks of nucleic acids, some like ATP and GTP, are additionally, cellular energy stores, others like cAMP or cGMP are messengers in signal transduction, and, yet others, such as FAD, NAD, thiamine phosphates and pyridoxal phosphate are cofactors for a range of enzymes [ 1 2 ] . Protein domains belonging to a relatively small set of structural folds are known to bind or catalyze reactions that utilize these organic phosphate compounds (see SCOP database: http://scop.mrc-lmb.cam.ac.uk/scop/) [ 3 4 ] . Several of these folds trace back to some of the earliest phases of the evolution of the protein world, and participate in a wide range of disparate biological functions in extant proteins [ 4 5 ] . Some folds, such as the P-loop fold, the Rossmann fold and the Hsp70-like fold, have been well studied, and comprise mainly of dedicated nucleotide binding or hydrolyzing proteins [ 6 7 8 9 ] . Others, such as the palm-domain, which is found in adenylyl cyclases and various nucleic acid polymerases, belong to more generalized protein folds that contain representatives with diverse biochemical activities [ 4 10 11 ] . Current availability of extensive genome sequence data, allows one to identify less numerous, nevertheless biological important organic phosphate-binding domains that may have previous eluded detection.

  • These could include controls such as direct microscopic counts of active and inactive bacteria [ 19 ] , extraction and quantitation of ATP [ 23 ] , extraction and analysis of fatty acids [ 24 ] , real-time PCR of universal 16S rDNA markers [ 25 ] , and/or use of a larger variety of culture media, including dilute organic media, in place of or in addition to plate count agar [ 26 ] . All of these approaches also have problems, such as the possible presence in soil of biological molecules that are not associated with living cells, the always-present inability of microbiologists to culture most of the bacteria present in soil, and the often unpredictable performance of PCR primers.

  • Partially purified fractions (reduced glutathione eluate) containing GSTCdc6 or GST showed an ACS binding activity in an ATP dependent manner.

  • A cocktail was added that contained 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.5 μM ubiquitin aldehyde in a volume of 15 ul.


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