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Example sentences for: atcop
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This finding is distinct from the observation for AtCOP1, which exists mainly as a homodimer [ 32 ] . In addition, the HsCOP1 complexes differ from the HsCOP9 signalosome complexes in size, as suggested by the different peak fractions (Fig 4).
GFP-N280 (amino acid 71-350), which contains the RING finger and the coiled-coil domain, but not the sequence corresponding to the NLS in AtCOP1, is also solely localized in the nucleus (Fig 5Aand 5B).
A partial cDNA clone homologous to AtCOP1 has been previously identified in mammals, containing all three conserved protein-protein interaction domains [ 20 ] . Remarkably, when expressed in plant cells, the mammalian COP1-reporter fusion protein exhibited a light-regulated nuclear localization pattern similar to AtCOP1 [ 20 ] . Although mammalian COP1 failed to rescue the Arabidopsis cop1 mutants, over-expression of the amino-terminal half of mammalian COP1 in Arabidopsis caused a dominant-negative phenotype, resembling the effect of over-expressing the corresponding fragment of AtCOP1 [ 20 21 ] .
The subcellular localization of AtCOP1 is regulated by light [ 14 ] , a feature that is critical for its function [ 4 29 ] . Interestingly, the localization of mammalian COP1 protein expressed in plant cells can also be regulated by light [ 20 ] , implicating a similar mechanism may operate for the nucleocytoplasmic partitioning of COP1 in mammalian cells.
Notably, the corresponding site in AtCOP1 is within the mapped cytoplasmic localization sequence [ 17 ] . Moreover, the NLS must also be located within amino acids 71 to 270 of MmCOP1, since NES-mutated or LMB-treated N200 is nuclear localized (Fig 6B).
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