Example sentences for: asynchronous

How can you use “asynchronous” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Since the background fluorescence in live, cycling cells was too high to derive conclusions about Rad26-GFP localization (data not shown), we prepared cells using a technique previously described in a study of fission yeast chromatin binding proteins [ 42 ] . An asynchronous population of cycling cells was first permeabilized using Zymolyase (Seikagaku Corp., Tokyo), then extracted with 1% Triton X-100 and finally fixed with methanol.

  • To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.

  • The facts that the parasite culture was asynchronous, i.e., contained all three major stages (ring, trophozoite, and schizont) (data not shown) and that the effect of dsRNA was severe, suggest that PfPP1 is required for cell cycle progression at all stages of the parasite.

  • You can't browse titles on a shelf if they're not there, and you can't drink café latte over an asynchronous connection.

  • The ongoing proliferation of HIV-1-infected podocytes following cell-cell contact led to the formation of multiple foci (Figure 2C) with intense, heterogenous nuclear cyclin D 1 immunofluorescence consistent with ongoing asynchronous proliferation in this population of cells (Figure 3).


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