Words similar to aspc-

Example sentences for: aspc-

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The pancreatic cell lines AsPc-1, CAPAN-1 and Panc-1 were purchased from the American Type Culture Collection (Manassas, VA) and were grown according to the manufacturer's instructions [ 28 29 30 ] . Genomic DNA was harvested from these cells using a Qiagen (Alameda, CA) Blood and Cell Culture DNA Midi Kit.
In contrast, in the TGFβ 1 -resistant AsPC-1 and Capan-1 cells, the interaction of mature TGFβ 1 with α V β 6 integrin resulted in an immobilization of high molecular weight proteins to the cytoskeleton without tyrosine phosphorylation (Figure 2).
In addition to the cell line Panc-1, K- ras 2 mutations in the second position of codon 12 were also shown to be present in the cell lines AsPC-1 (G → A; data not shown) and CAPAN-1 (G → T; data not shown).
The cell lines Capan-1 and AsPC-1 are not only resistant to TGFβ 1 because of their alterations in the SMAD pathway, but also because they cannot complete the alternate pathway, as demonstrated above.
Adherence to mutational hotspots was directly responsible for the inability to detect the p53 mutations in the CAPAN and AsPc-1 cell lines [ 19 21 ] . The best approach for detecting mutations outside the hotspots will involve direct sequencing of the polony PCR products [ 2 13 14 ] .