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6Ashows, a synthetic β-box peptide competed for binding to N-CoR, albeit somewhat less efficiently than native GRIP1 NR box 2. Similar results were obtained in competition experiments that used GST-GRIP1 instead of GST-N-CoR (data not shown).
1Ashows Southern blots from DNA of control (+/+), γ 1 het (+/-) and γ 1 null (-/-) mice, after digestion with Eco RI and hybridization to probe 1. The γ 1 het and γ 1 null mice show the predicted 12.
Figure 5ashows an immunoprecipitation (anti-GFP) plus protein blot (anti-Ptc) analysis of extracts from these cells treated for 4, 8 and 24 hours with vehicle, 25 nM Hh protein or 0.2 μM Hh agonist (see Figure 1e; Hh-Ag 1.2).
Figure 4Ashows that the GFP positive population of Ad-transduced cells had a comparable proliferative response to the non-transduced GFP negative cells across the entire antigen dose range.
1Ashows that pAD-LeFPP leads to activation of the HIS gene in combination with pBD-LeMAF1 (sector a) and with pBD-AtMAF1 (sector b), but not with pBDAtRanGAP1 (sector c) or the binding domain vector pBD-GAL4 alone (sector d).