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Example sentences for: ase

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Multiple sequence alignments of diverse intein sequences identified eight motifs composed of moderately conserved residues [ 18 28 ] . The A-ATPase A subunit of the Thermoplasma and Pyrococcus intein multiple sequence alignment (with manual modification) is shown in figure 1. The T. acidophilum intein (173 amino acids long) is among the shortest inteins known, and the alignment with other inteins reveals the absence of sequences homologous to the typical endonuclease motifs.
Gimble and Thorner [ 14 ] demonstrated intein homing in Saccharomyces cerevisiae using engineered V-ATPase genes from which the intein encoding portion had been previously removed.
Here we present data on the cloning and expression of the T. acidophilum A-ATPase A subunit in E. coli, and we discuss implications for the location, propagation and distribution of inteins among organisms.
One explanation for the discrepancy between phylogenetic classification and distribution of the A-ATPase intein is horizontal gene transfer: the intein was not present in the last common ancestor of Thermoplasma and Pyrococcus, rather the intein invaded one of the lineages after their split and was more recently horizontally transferred to the other lineage.
Either the whole A-ATPase catalytic subunits was transferred, or the intein alone spread as a selfish genetic element.