Words similar to ascites
Example sentences for: ascites
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Mouse IgG 1 and IgG 2a standards were purified by sepharose-coupled protein G from irrelevant (non-proteoglycan-specific) monoclonal antibody-containing ascites fluids and coated directly onto the microplate's surface.
Lysophophatidic Acid (LPA) is a member of this class of compounds, and its plasma levels have been proposed as a potential biomarker for ovarian cancer [ 16 17 ] . LPA is a family of related molecules with molecular weights in the vicinity of 400 to 600 Daltons, and a variety of LPA species has been reported to be increased in malignant ascites from patients with ovarian cancer as detected by electrospray ionization mass spectrometry (ESI-MS) [ 18 ] . LPA related species have also been reported to be increased in plasma samples from patients with ovarian cancer using a combination of thin layer chromatography (to isolate an "LPA band" from patient plasma) followed by ESI-MS.
We used both antibodies in the form of ascites fluid (1 mg/ml), at 10 μg/ml dilution for immunohistochemistry and 1 μg/ml for western blot analysis (4°C overnight).
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
10 TCR) were purified from ascites and conjugated with fluorescein isothiocyanate (FITC), biotin or phycoerythrin (PE) by Dr. Roger Lallone (Brookwood Biomedical; Birmingham, AL).
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