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Example sentences for: asbt

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8 and CHO.asbt.
To determine if non-liver derived cells also posses differential sensitivity to bile acid-induced cytotoxicity, we engineered CHO cells to take up bile acids actively by expressing asbt.
Correspondingly, in CHO.asbt lysates, an immunoreactive band of comparable size (~45 kDa) to rat asbt protein can be easily detected using an anti-asbt antibody (Figure 3B).
To establish cell lines stably expressing asbt, a cDNA encoding the hamster asbt [ 36 ] was cloned into pBK-CMV expression vector (Stratagene, La Jolla, CA).
Colonies surviving the selection process were assayed for asbt activity and several clones showing a range of bile acid uptake activities were selected for further analysis.