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Example sentences for: asbt
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Members of the caspase family of cysteine proteases have been implicated in the initiation and execution phase of apoptosis [ 24 ] . In addition, several studies have shown the activation of the caspase cascade by toxic bile acids in hepatocytes [ 25 26 ] . We thus measured caspase activity after bile acid treatment in CHO.asbt.
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
Furthermore, it was possible to chase the incorporated radiolabeled TCA from CHO.asbt.
Colonies surviving the selection process were assayed for asbt activity and several clones showing a range of bile acid uptake activities were selected for further analysis.
Activation of caspase 8 was detected in CHO.asbt.