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Example sentences for: arrayoligoselector

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Subsequent versions of ArrayOligoSelector were improved by calculating a theoretical energy of binding between the oligonucleotide and its most probable cross-hybridization target in the genome ('second best target').
The initial setting of the GC content filter was set to 28% GC (73°C T m . Subsequently, with the release of additional sequence information, a new set of predictions was generated in April 2002 and an additional 1,025 oligonucleotides were selected using the upgraded version of ArrayOligoSelector.
ArrayOligoSelector automatically detects such sequences by subjecting candidate oligonucleotide sequences to a lossless compression [ 36].
To avoid selecting oligonucleotides with secondary complex structure, ArrayOligoSelector uses the Smith-Waterman algorithm with the PAM47 DNA matrix to calculate the optimal alignment score between the candidate oligonucleotide sequence and the reverse complement of that sequence [ 24].
To evaluate the potential for cross-hybridization, early versions of ArrayOligoSelector used a simple BLASTN alignment identity [ 29].