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Example sentences for: arna

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Three micrograms of aRNA (unless otherwise specified) or 2 μg poly(A) +RNA were labelled either with Cy5-dUTP (experimental sample) or Cy3-dUTP (reference) in a 30.
aRNA was purified using Mini RNA Isolation Kit or RNA Clean-up kit (Zymo Research), eluting in 2 × 8 μl RNase-free water.
Each probe/test sample (antisense RNA (aRNA) obtained at each time point) was labeled with Cy5 and mixed with Cy3-labeled reference sample (non-stimulated PBMC obtained from apheresis before IL-2 administration).
Nevertheless, the good correspondence of the hybridizing spots shows that the aRNA is an essentially complete representation of the population of transcripts present in the initial pool.
In conclusion, T7 based linear amplification generates high fidelity aRNA for gene expression profiling using high-density cDNA microarrays.