Words similar to aprotinin
Example sentences for: aprotinin
How can you use “aprotinin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Cells were suspended in buffer containing 50 mM HEPES pH 7.4, 10 mM KCl, 1 mM EDTA, 1 mM DTT, 1 μg/ml pepstatin A, 1 μg/ml leupeptin, 10 μg/ml soybean trypsin inhibitor, 10 μg/ml aprotinin and 0.5% NP-40 and mixed periodically using a vortex.
Briefly, retinae were isolated with homogenization buffer (HB, 0.32 mM sucrose, 2 mM Hepes, pH 7.4, 1 mM EGTA, 2 μg/ml leupeptin and 2 μg/mg Aprotinin) and homogenized.
For example in vitro addition of plasmin, a serine protease, accelerates the migration of neuroblastoma cells through a matrigel base by a factor of five [ 7 ] . The subsequent addition of aprotinin, a plasmin inhibitor, decreases the migratory population to the same degree [ 8 9 ] . Furthermore, Seeds et al.
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
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