Words similar to apn

Example sentences for: apn

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Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
Hybrid toxin 4109 is particular useful in this context, because it is comprised of domains I and II from Cry1Aa and domain III from Cry1Ac (Aa/Aa/Ac) [ 3 ] . Hybrid-toxin 4109 binding to both receptors was not noticeably different from Cry1Aa: for APN binding, k a = 1.9 × 10 4M -1s -1(+/- 1.4 × 10 2), k d = 1.5 × 10 -3s -1(+/- 1.6 × 10 -5), and K D = 78 nM; for cadherin binding, k a = 1.3 × 10 4M -1s -1(+/- 2 × 10 2), k d = 3.34 × 10 -5s -1(+/- 2 × 10 -5), and K D = 2.6 nM.
A protein with APN enzymatic activity eluted 75 minutes after injection (Fig.
Nonetheless, Cry1Aa is 210 times more toxic than Cry1Ac to B. mori [ 4 ] . As a whole, B. mori APN research indicates the presence of at least three genetic isoforms [ 7 11 12 ] , with toxin affinities ranging from nanomolar to none at all.
This apparent off-rate clearly accounted for Cry1Aa's higher affinity for cadherin than for APN.