Example sentences for: aph

How can you use “aph” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.

  • One reporter gene, the aph gene which confers resistance to kanamycin, allows for selection of active promoters and a second reporter gene, the xylE gene which encodes catechol 2,3 dioxygenase, allows for screening for loss of promoter activity.

  • 4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.

  • These differ for Anabaena and Nostoc as depicted in Figure 2. Anabaena has genes encoding aph and a hypothetical gene (open reading frame (ORF)) inserted between aroB and the trpAa • trpAb fusion.

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.


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