Words similar to aph
Example sentences for: aph
How can you use “aph” in a sentence? Here are some example sentences to help you improve your vocabulary:
These differ for Anabaena and Nostoc as depicted in Figure 2. Anabaena has genes encoding aph and a hypothetical gene (open reading frame (ORF)) inserted between aroB and the trpAa • trpAb fusion.
The aph gene encodes an uncharacterized protein of the defined alkaline phosphatases (metalloenzyme superfamily) (group COG1524 in the COGS database).
The two PCR products were mixed, allowed to anneal, and amplified using two primers, one (1915) complementary to the 5' end of the aph gene and a second (1918) complementary to the 3' end of xylE to produce a 1785-bp fragment.
Human Fhit [ 14 ] and the S. cerevisiae Fhit homolog [ 13 ] , which was called Aph1 but is here termed Hnt2 under nomenclature aproved by the Saccharomyces Genome Database, cleave ApppA more readily while Aph1, the S. pombe homolog, cleaves AppppA more readily [ 20 ] . Consistent with the ApppA hydrolase activity of purified Fhit protein, most cancer cell lines that are Fhit negative at the protein level have higher levels of ApppA than cell lines that are Fhit positive [ 21 ] . Nonetheless, the actual concentrations of dinucleoside polyphosphates were submicromolar in every cell culture sample [ 21 ] and thus, under the reported culture conditions, the measured dinucleoside polyphosphates would not be expected to occupy the Fhit active site substantially [ 19 ] . Dinucleoside polyphosphate levels were measured in adenine-requiring S. cerevisiae strains before or after disruption of the Fhit-homologous HNT2 gene [ 13 ] and in adenine-requiring S. pombe strains as a function of disruption and overexpression of the Fhit-homologous aph1 gene [ 22 ] . Recently, it was observed that diadenosine polyphosphates undergo a divalent cation-dependent conformational change that might mediate their biosynthesis, catabolism or signaling properties [ 23 ] .
pCL8 is a derivative of pCL5 [ 9 ] and contains the luciferase ( luc ) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene ( aph ) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the P
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