Words similar to aph
Example sentences for: aph
How can you use “aph” in a sentence? Here are some example sentences to help you improve your vocabulary:
Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.
One reporter gene, the aph gene which confers resistance to kanamycin, allows for selection of active promoters and a second reporter gene, the xylE gene which encodes catechol 2,3 dioxygenase, allows for screening for loss of promoter activity.
4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
These differ for Anabaena and Nostoc as depicted in Figure 2. Anabaena has genes encoding aph and a hypothetical gene (open reading frame (ORF)) inserted between aroB and the trpAa • trpAb fusion.
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.