Words similar to aph

Example sentences for: aph

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4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
These differ for Anabaena and Nostoc as depicted in Figure 2. Anabaena has genes encoding aph and a hypothetical gene (open reading frame (ORF)) inserted between aroB and the trpAa • trpAb fusion.
One reporter gene, the aph gene which confers resistance to kanamycin, allows for selection of active promoters and a second reporter gene, the xylE gene which encodes catechol 2,3 dioxygenase, allows for screening for loss of promoter activity.
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.
pCL8 is a derivative of pCL5 [ 9 ] and contains the luciferase ( luc ) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene ( aph ) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the P