Words similar to aph
Example sentences for: aph
How can you use “aph” in a sentence? Here are some example sentences to help you improve your vocabulary:
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.
Human Fhit [ 14 ] and the S. cerevisiae Fhit homolog [ 13 ] , which was called Aph1 but is here termed Hnt2 under nomenclature aproved by the Saccharomyces Genome Database, cleave ApppA more readily while Aph1, the S. pombe homolog, cleaves AppppA more readily [ 20 ] . Consistent with the ApppA hydrolase activity of purified Fhit protein, most cancer cell lines that are Fhit negative at the protein level have higher levels of ApppA than cell lines that are Fhit positive [ 21 ] . Nonetheless, the actual concentrations of dinucleoside polyphosphates were submicromolar in every cell culture sample [ 21 ] and thus, under the reported culture conditions, the measured dinucleoside polyphosphates would not be expected to occupy the Fhit active site substantially [ 19 ] . Dinucleoside polyphosphate levels were measured in adenine-requiring S. cerevisiae strains before or after disruption of the Fhit-homologous HNT2 gene [ 13 ] and in adenine-requiring S. pombe strains as a function of disruption and overexpression of the Fhit-homologous aph1 gene [ 22 ] . Recently, it was observed that diadenosine polyphosphates undergo a divalent cation-dependent conformational change that might mediate their biosynthesis, catabolism or signaling properties [ 23 ] .
These differ for Anabaena and Nostoc as depicted in Figure 2. Anabaena has genes encoding aph and a hypothetical gene (open reading frame (ORF)) inserted between aroB and the trpAa • trpAb fusion.
The two PCR products were mixed, allowed to anneal, and amplified using two primers, one (1915) complementary to the 5' end of the aph gene and a second (1918) complementary to the 3' end of xylE to produce a 1785-bp fragment.
4 (Figure 4) is a promoter-trap plasmid containing a promoterless aph gene and xylE gene, an E. coli rRNA transcriptional terminator upstream of the reporter genes, an oriM for plasmid replication in M. smegmatis , an oriE for plasmid replication in E. coli , and a hyg gene for selection in E. coli and M. smegmatis bacteria.
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