Example sentences for: aph

How can you use “aph” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pCL8 is a derivative of pCL5 [ 9 ] and contains the luciferase ( luc ) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene ( aph ) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the P

  • First, genomic DNA from the sur2 clone was digested with Pst I, and genomic DNA from the sur3 clone was digested with Bam HI to generate fragments of each integrated cosmid carrying oriE, aph, and a portion of the M. tuberculosis genomic DNA insert.

  • In the case of disruption of the Fhit and Hnt2-homologous aph1 gene in S. pombe , which encodes an enzyme relatively specific for a AppppA [ 20 ] , a 290-fold increase in AppppA concentration was observed [ 22 ] . Our data indicate that Hnt2 hydrolyzes ApppN and AppppN in vivo in budding yeast and that an intact adenine biosynthetic pathway is required for high-level synthesis and accumulation of adenylylated dinucleoside polyphosphates.

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.

  • Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.


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