Example sentences for: anti-β-actin

How can you use “anti-β-actin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5% Tween 20, 1% milk) and incubated with primary antibody for 45 min at 37°C (1:1500 anti-eNOS mono [BD Transduction Laboratories, San Diego, CA, USA]; 1:1500 anti-nNOS poly [Zymed Laboratories, South San Francisco, CA, USA]; 1:200 anti-iNOS poly [Affinity Bioreagents, Golden, CO, USA]; and 1:1500 anti-β-actin mono [Sigma A5441; Sigma-Aldrich, St Louis, MO, USA]).

  • The blots were first blocked with 5% non-fat dry milk in TPBS buffer (PBS with 0.5% Tween 20) at RT for 1 hr, and then incubated with 1:3000 dilution of anti-β-actin (Sigma, St. Louis MO), 0.75 μg/ml of anti-NR1 (Chemicon, Temecula, CA), 1:1000 dilution of anti-NR2A (Chemicon), 0.35 μg/ml of anti-NR2B (Chemicon) and 1 μg/ml of anti-GluR1 (Chemicon) antibodies, respectively, at 4°C overnight.

  • Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.

  • Anti-β-actin monoclonal antibody was purchased from Sigma.


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